ALTERATION OF NUCLEAR AND NUCLEOLAR PYROANTIMONATE-OSMIUM REACTIVITY BY GLUTARALDEHYDE FIXATION
نویسندگان
چکیده
منابع مشابه
Nuclear Precipitates in Pyroantimonate-osmium Tetroxide-fixed Tissues
Recent biochemical studies indicate a significant role for sodium ions in nuclear metabolism. Protein and nucleic acid syntheses in the nucleus depend upon sodium, apparently through a stimulating effect of this cation on the transport of amino acid, purine, and pyrimidine into the nucleus (1). The nuclei of avian erythrocytes and calf thymocytes appear to contain higher sodium concentrations t...
متن کاملPerfusion fixation with glutaraldehyde and post-fixation with osmium tetroxide for electron microscopy.
The conductivity of cerebral cortex drops during perfusion with glutaraldehyde in 5 min to about 60% of the original value, to remain unchanged during the subsequent 10-15 min of perfusion. Circulatory arrest causes a similar drop in the tissue conductivity. Perfusion of asphyxiated tissue with glutaraldehyde does not produce additional major changes in the conductivity. Perfusion of the cortex...
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Fixation by osmium tetroxide and glutaraldehyde of zymogen granules isolated from rat parotid and pancreas was investigated. Protein determinations showed that osmium tetroxide caused rapid release of most of the soluble protein of the granule during fixation in buffered isotonic sucrose. Such granules when examined in the electron microscope after shadow casting appeared quite flat, indicating...
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Human leukocytes in suspension or in monolayer cultures have been processed for electron microscopy by fixation in a freshly made cold mixture of glutaraldehyde and osmium tetroxide and by "postfixation" in uranyl acetate. Simultaneous exposure to glutaraldehyde and osmium tetroxide eliminates many of the shortcomings seen when either of these agents is used alone as the initial fixative. Speci...
متن کاملQuantitation of Human Red Blood Cell Fixation by Glutaraldehyde
The uptake of glutaraldehyde by human red blood cells has been measured as a function of time by a freezing point osmometer. The rate of attachment of glutaraldehyde to the cell proteins is high over the first hour, declining to zero over a period of a few days. The number of glutaraldehyde molecules cross-linking with each hemoglobin molecule is of the order of 200, in reasonable agreement wit...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1971
ISSN: 0022-1554,1551-5044
DOI: 10.1177/19.6.388